Analysis of NGS data

• Transcriptome analysis using RNA-seq (mRNA, lncRNA, medium-sized RNA, smallRNA)
• Translation control using Ribo-seq
• Binding analyzes using ChIP- and CLIP-seq
• Genome analysis using Whole Genome-seq or Exom-seq
• Oxford Nanopore DNA/RNA-seq data

• Quality control of raw data
• Removal of adapter sequences and quality trimming
• Mapping (assignment of reads to known reference genomes)
• Annotation (assignment of mapped reads to known genes)
• Determination of read coverage
• Normalization (regarding RNA composition, transcript length, differential expression etc.)

• Determination of differentially expressed genes
• Correlation of mRNA to miRNA expression (incl. target analysis)
• Determination of the expression of alternative isoforms (e.g. due to alternative splicing)
• Quantification of translation efficiency (Ribo-seq)
• Analysis of binding profiles (ChIP- and CLIP-seq)
• Determination of effector pathways using gene annotation enrichment analysis (GAEA) and gene set enrichment analysis (GSEA)
• Metagene analyses, inclusion of "public data" (e.g. TCGA) and further analyzes on request

List of fees for the evaluation and further analysis of NGS data on known genomes.

 ¹ Per started billing unit.

Data retention for 3 months.